PerfectSil Columns

Which PerfectSil column should I start with for small molecules?

For most small-molecule methods, begin with a reversed-phase ODS/C18-type chemistry in an 80 Å pore material. Then choose length and particle size based on your resolution and instrument pressure limits.

How do I choose between 4.6 mm and 2.1 mm ID columns?

Use 4.6 mm for maximum robustness and compatibility. Use 2.1 mm to reduce solvent use and improve sensitivity, especially with LC-MS, provided your system has low extra-column volume.

What does pore size (80 Å vs 300 Å) change?

80 Å is typically optimized for small molecules. 300 Å is designed for larger molecules such as peptides/proteins where larger pores improve access and recovery.

Can you help with method transfer from another brand?

Yes. Share your current column dimensions, chemistry, particle size, and method conditions in the enquiry form. We will recommend the closest PerfectSil configuration and scaling guidance.

Why am I seeing peak tailing with basic analytes?

Tailing can be caused by silanol interactions, sample solvent mismatch, or contamination. A properly deactivated/end-capped phase plus optimized pH and sample solvent usually improves symmetry.

Do you support LC-MS applications?

Many PerfectSil phases are suitable for LC-MS workflows; choose volatile mobile phases and avoid non-volatile buffers. Share your detector details for the best match.